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2011-08-23 14:39:36 UTC
Objective
Ambitious graduate with strong laboratory skills seeking internship.
Education
Bachelor of Science in Biology, May 2010
Saint Joseph's College Of Maine
Qualifications
Strong background in laboratory procedures. Proficiency with ELISA, Western blot, gene sequencing, and a variety of other lab techniques. Ability to manage numerous projects simultaneously. Willing to learn new techniques and take courses.
Laboratory Skills
Genetics:
· Isolated Human DNA from cheek cells and used PCR amplification to copy DNA for sequencing. Used Gene sequencing to identify specific sequences.
· Isolated bacterial DNA and identified species of unknown bacterial colonies using PCR and universal 16sRNA primers. BLAST database was used for identification.
· Used Gel Electrophoresis to analyze Bovine and Swine DNA.
Microbiology
· Frequently performed Gram stains and Acid fast stains to observe bacterial morphology.
· Used various types of media for bacterial growth, transport, and identification. Selective and differential media were used along with various environmental conditions (anaerobic, aerobic, high and low saline conditions, and high and low temperature) to identify unknown bacterial colonies.
· Isolated and identified bacteria from various sources that include throat cultures and urine samples.
Bioassay
· Experienced with lab techniques including PCR, DNA isolation, DNA amplification, DNA sequencing, DNA analysis, Western Blot, Southern blot, Enzyme-linked immunosorbent assay (ELISA), bacterial isolation and identification techniques, electrophoresis, and BLAST (Basic local alignment search tool)
Cell Biology
· Performed experiments consisting of PH gradients, Amino acid analysis, and the procedure of electrophoresis.
Developmental Biology
· Artificially inseminated leopard frog eggs and observed all stages of embryogenesis.
· Cut a viewing hole in a fertilized chicken egg and observed development of the embryo until 42 hours when the embryo was then removed and structures were observed under a microscope.
Relevant Research Experience
Federal Grant Research position: May 09 - August 09
Saint Joseph's College in coordination with UNE and USM
Saint Joseph's College of Maine
278 Whites Bridge Road
Standish, Maine 04084
Phone: 207.893.7757
· Synthesized novel Siloles and related fluorescing Metalol compounds. Research included synthesis, isolation and purification, analysis and nomenclature. Presentation of weekly reports on progress were required.
· NMR, Fluorescent spectroscopy, and Mass spectrometry were used frequently to determine the chemical makeup and purity of the product.
· High performance Liquid chromatography was used to help purify and identify the novel compounds.
· Synthesis included the use of dangerous chemicals such as Silicon tetrachloride, Germanium tetrachloride, and Tin tetrachloride. Careful preparation and observation of safety were necessary under anhydrous conditions with a continuous flow of Argon/Nitrogen gas.
Computer Skills
MS PowerPoint, MS Excel, MS Word, Photoshop CS, Dreamweaver CS5